Journal: bioRxiv

Article Title: Sialidases derived from Gardnerella vaginalis remodel the sperm glycocalyx and impair sperm function

doi: 10.1101/2025.02.01.636076

Figure Lengend Snippet: A) Schematic describing lectin flow cytometry and representative glycan epitopes. B, C, D) Sperm were exposed to sialidases (AUS 20 units or GvNanH2 160 units) for 1 hour, fixed in formalin, and stained with MAL-II-biotin/Neutravidin-Alexa Fluor 488, SNA-Cy5, or PSA-FITC respectively. Each point represents the median fluorescence intensity from one donor. Statistics represent a mixed model comparison with post-hoc Holm-Šidák tests. E) Schematic representing change in zeta potential after removal of negatively charged sialic acid on sperm surface. F) Sperm were treated with sialidase at various doses for 1 hour, and zeta potential was measured via dynamic light scattering. Each measurement represents the average of seven technical replicates, and statistics represent a mixed model comparison with post hoc Holm-Šidák tests.

Article Snippet: Biotinylated Maackia Amurensis Lectin II (MAL II, Cat #:B-1265-1) and Cy5-conjugated Sambucus Nigra Lectin (SNA, Cat #: CL-1305-1) were purchased from Vector Labs. Human Contraception Antibody (HCA), was a gift from ZabBio.

Techniques: Flow Cytometry, Staining, Fluorescence, Comparison, Zeta Potential Analyzer

Journal: Scientific Reports

Article Title: Tulane virus recognizes sialic acids as cellular receptors

doi: 10.1038/srep11784

Figure Lengend Snippet: ( A ) Sambucus nigra lectin (SNL) inhibited, while Maackia amurensis leukoagglutinin (MAL) increased TV infectivity in LLC-MK2 cells. Prior to TV infection, the LLC-MK2 cells were treated with SA-binding lectins; 100 μg/mL of SNL or MAL in 1x phosphate buffer saline (PBS, pH7.4). Mock control cells were treated with PBS only. The mean values (plaque numbers) of the mock/control group were set as 100%, while percentages (%) of the plaque numbers for individual wells of both mock/control and neuraminidase-treatment groups relative to the mean values of the mock/control group were calculated and shown. The statistical differences between data groups are indicated. Each experiment was repeated at least eight times. ( B and C ) Dose-response effects of SNL and MAL at different concentrations on TV infectivity in LLC-MK2 cells. The mean value (plaque numbers) of the control group without SNL ( B ) or MAL ( C ) treatment (0 μg/mL) was set 100%, while the reduction ( B ) or enhancement ( C ) rates (%) of the SNL/MAL treatment groups were calculated and shown. Each experiment was repeated at least four times. The statistical differences between the control groups (0 μg/mL SNL/MAL) and the SNL/MAL treatment groups are indicated. “ns”, “*”, “**”, and “***” indicate P values > 0.05, <0.05, <0.01, and <0.001, respectively.

Article Snippet: The role of cell surface SAs on TV infectivity in LLC-MK2 cells were assessed following treatment with two commonly used SA-binding lectins, Sambucus nigra lectin (SNL) or Maackia amurensis leukoagglutinin (MAL).

Techniques: Infection, Binding Assay, Saline, Control